RISE - Correlative Raman Imaging and Scanning Electron Microscopy

  • World's first fully-integrated Raman Imaging and Scanning Electron Microscopes

    RISE Microscopy is a novel correlative microscopy technique that combines SEM and confocal Raman Imaging. Through RISE Microscopy ultra-structural surface properties can be linked to molecular compound information.


    The RISE Microscope combines all features of a stand-alone SEM and the confocal Raman imaging microscope alpha300 within one instrument:

    • Quick and convenient switching between Raman and SEM measurement

    • Automated sample transfer from one measuring position to the other

    • Integrated software interface for user-friendly measurement control

    • Correlation of the measurement results and image overlay

    • No compromise in SEM and Raman imaging capabilities

  • WITec RISE Hematite

The RISE Technique

  • Technique RISE 072015
  • For RISE microscopy samples are automatically transferred from one measuring position to the other within the vacuum chamber of the SEM, streamlining the workflow and drastically improving the instrument's ease of use.

Application Examples

  • RISE Raman SEM Brain Tissue
    Raman-SEM image overlay of a hamster brain tissue sample. Color-coded Raman image parameters: Green: White brain matter; Red: Gray brain matter; 100 µm x 100 µm, 300 x 300 pixels = 90,000 spectra, 50 ms integration time per spectrum.
  • RISE Raman SEM GaAs
    Raman-SEM image overlay of a GaAs semiconductor sample. Color-coded Raman image parameters: Yellow: Gold substrate; Red: GaAs; Blue: Residues from production; 50 µm x 50 µm, 300 x 300 pixels = 90,000 spectra, 34 ms integartion time per spectrum.
  • RISE Raman SEM Polymers
    Raman-SEM image overlay of a PMMA-PS polymer blend- Color-coded Raman image parameters: Green: Polystyrene; Red: Polymethyl methacrylate.
  • RISE Raman SEM Diorite
    RISE Microscopy and EDX analysis of a geological sample. Left: Overlaid SEM-EDX image: Three different element groups can be distinguished (Orange: Si, O; Purple: Si, Al, Fe, Ca; Green: Na). Middle: The Raman-SEM image overlay of the same sample area shows the distribution of the molecular compounds. Right: Corresponding Raman spectra. Red: Epidote; Green: Quartz; Brown: Plagioclase (Albite); Plus additional molecular compounds. 

RISE Key Features

    • Scanning Electron Microscopy:

    • Technique SEMall
      Scanning Electron Microscopy features a variety of analysis techniques. With RISE, the chemistry of molecular compounds can be imaged additionally.
  • Confocal Raman Microscopy:

    • Confocal Raman Imaging with unprecedented performance in speed, sensitivity, and resolution
    • Hyperspectral image generation with the information of a complete Raman spectrum at every image pixel
    • Excellent diffraction limited lateral resolution of 200 - 300 nm
    • Outstanding depth resolution ideally suited for 3D image generation and depth profiles
    • Ultrahigh-throughput lens-based spectroscopic system for highest sensitivity and best performance in spectral resolution
    • Ultra-fast Raman imaging option with only 0.76 ms integration time per spectrum
    • Non-destructive imaging technique: no staining or fixation of the sample required

RISE Microscopy is suitable for you...

  • a Raman newcomer you will benefit from the ease-of-use and intuitive measurement procedure an experienced user you will appreciate the exceptional correlative microscope performance with the advantages of both techniques included in one instrument

    Materials sciene, nanotechnology, polymer science, geoscience, life science, pharmaceutical industry...

    ...regardless of the field in which you are working, RISE Microscopy will empower you with its unique imaging capabilities

Award winning technology

  • RISE Microscopy has been recognized with a 2015 Photonics Prism Award. An expert jury named the correlative RISE microscope as winner in the metrology category. The Prism Award is given for top innovations in the field of photonics, granted by Photonics Media and sponsored by the international Society for Optics and Photonics (SPIE).

  • DrkGreenWinnerBox



  • Please fill in all data fields to ensure we can process your inquiry as quickly as possible.

    Privacy Protection Statement:

    The personal information collected through this contact form will be used to respond to your submitted query. WITec and, if applicable, a WITec partner company responsible for processing the inquiry may contact you by e-mail, phone or mail for marketing purposes. The processing of your data is based on our data protection regulations. You may withdraw permission to use your data at any time.