Correlative Microscopy Techniques

Correlative Microscopy Techniques

  • The modular design of the WITec microscope systems allows to combine various imaging techniques such as Raman imaging, fluorescence, luminescence, atomic force microscopy (AFM), near-field microscopy (SNOM or NSOM) and scanning electron microscopy in one single instrument without compromising the analyzing and imaging quality.

    The integrated WITec software interface enables user-friendly measurement control, quick and easy correlation of the results and image overlay.

  • WITec Correlative Raman Fluorescence Cell Biology
    Correlative Raman-fluorescence microscopy on a biological cell. Green: GFP fluorescence; yellow, pink and white: Raman signal of nucleus, nucleoli and lipids, respectively.

Raman-SEM (RISE)

  • The Raman-SEM (RISE) combination is a unique correlative microscopy technique provided by WITec. Structures in the nm-range detected by Scanning Electron Microscopy (SEM)  can be correlated to chemical Raman imaging from the same sample area.

    More information about WITec's RISE Microscope

  • WITec Correlative Raman SEM TiO2
    Correlative Raman-SEM (RISE) image of TiO2 particles. The Raman image is overlaid on the SEM image. Different modifications of TiO2 were analyzed by Raman microscopy (blue: anatase, red: rutile).


  • By combining confocal Raman imaging with AFM, the chemical composition of the sample can be linked with the surface characteristics. These two complementary techniques are available in combined Raman-AFM WITec microscopes for flexible and comprehensive sample characterization.

    More information about WITec's Raman-AFM Microscope alpha300 RA

  • WITec Correlative Raman AFM Polymer Blend
    Correlative Raman-AFM image of a polymer blend. Left: Topographic AFM image. Right: Raman image. Red, blue and green: different polymers of the blend.